This research outlines a comprehensive study aimed at assessing water quality in the Wekiva River basin, focusing specifically on nitrate (NO3) ranging from 0.01 to 2 and phosphate (PO4) ranging from 0.03 to 0.5 concentrations. The study incorporates field measurements of dissolved oxygen, specific conductance, and pH to provide an overview of the aquatic ecosystems. The colorimetric analysis method was employed, involving the addition of chemical reagents to water samples, enabling the detection of nitrate (indicated by an amber color) and phosphate (indicated by a blue color). Given the unique tea-colored characteristic of the Little Wekiva River, the research primarily concentrates on collecting samples from the Wekiva River and its springs, known for their natural clarity. While the St. Johns River Water Management District (SJRWMD) conducts nitrate and phosphate analyses in these areas, this research contributes unique insights by collecting samples from previously unsampled locations, aiming to enhance the understanding of the local aquatic ecosystem and surroundings contributing to such changes. To optimize data collection efforts, an online search identified existing sampling sites and data, enabling the research team to pinpoint locations for their work. The research team expanded the scope beyond SJRWMD's sampling areas, collecting and analyzing samples from Wekiwa Springs down past Wekiva Island (first 1.5 miles) to assess spatial variability in a single day. This research seeks to provide valuable data to better monitor and manage water quality in the Wekiva River basin, ultimately aiding in the preservation and protection of this vital ecological resource.